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| NISHIMOTO, Hiromi; HAMANO, Seizo; HILL, Glen Alan; MIYAMOTO, Akio; TETSUKA, Masafumi; 宮本, 明夫; 手塚, 雅文. |
| A simple and clear means to identify the physiological status of follicles is essential for study of follicular biology. In the present study, we verified a novel classification procedure based on analysis of the follicular population and glucose concentration in follicular fluid (FF) as an alternative method to classify bovine follicles. Paired ovaries were collected from heifers, and the number of follicles and stage of the CL were recorded. Follicles were initially divided into the following 3 groups according to diameter and the ratio of E2 and P4 (E/P): E2 active (E-A: E/P≥1), E2 inactive (E-I: E/P<1, ≥8.5 mm) and small follicles (E/P<1, <8.5 mm). E-A follicles were easily identified as E2-rich dominant follicles and were further classified... |
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Palavras-chave: Bovine; Follicular fluid; Follicle classification; Glucose; Lactate; Steroid. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2828 |
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| TETSUKA, Masafumi; NISHIMOTO, Hiromi; MIYAMOTO, Akio; OKUDA, Kiyoshi; HAMANO, Seizo; 手塚, 雅文; 宮本, 明夫. |
| Glucocorticoids modulate ovarian function in cattle. However, their regulatory mechanisms have not been fully elucidated. In the present study, we examined gene expression of two glucocorticoid-metabolizing enzymes, a bidirectional 11β-HSD type 1 (11HSD1) and a dehydrogenase 11β-HSD type 2 (11HSD2), and glucocorticoid receptor (GR) in bovine follicles during follicular maturation and atresia. Granulosa cells (GCs) and theca interna layers (TIs) were harvested from follicles classified as small growing, dominant, preovulatory, early atretic and late atretic follicles. The expression levels of 11HSD1, 11HSD2 and GR mRNA were quantified by real-time PCR. In the healthy follicles, expression of 11HSD1 mRNA increased as follicles matured, both in GCs and TIs. A... |
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Palavras-chave: Bovine; Cortisol; 11β-HSD; Follicle; Glucocorticoid receptor; MRNA. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2810 |
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| MURASAWA, Maya; TAKAHASHI, Tsuyoshi; NISHIMOTO, Hiromi; YAMAMOTO, Sawa; HAMANO, Seizo; TETSUKA, Masafumi; 手塚, 雅文. |
| The size of the ovary varies substantially among cattle. This variation may influence the potential of the ovary to produce follicles. In the present study, we examined whether a relationship exists between the weight of the ovary and the number of antral follicles ≥1 mm. Paired ovaries were obtained from Holstein × Japanese Black F1 heifers. Follicles were classified into three size categories (small: 1.0 - < 5.0 mm, medium: 5.0 - < 8.5 mm and large: ≥ 8.5 mm), and the number of follicles in each category was recorded. Large variations in the weight of ovaries and the number of follicles were observed among animals. Significant positive correlations (r≥0.4, P<0.001) were found between the weight of intact ovaries and the number of follicles in... |
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Palavras-chave: Cattle; Follicular development; Follicular population; Ovarian weight. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3035 |
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